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SNCB Polyclonal antibody

SNCB Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10498-1-AP

Synonyms

Beta synuclein, SNCB, synuclein, beta

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SNCB Polyclonal antibody (10498-1-AP)

    WB analysis of mouse brain using 10498-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SNCB Polyclonal antibody (10498-1-AP)

    WB analysis of mouse brain using 10498-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SNCB Polyclonal antibody (10498-1-AP)

    WB analysis of mouse brain using 10498-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SNCB Polyclonal antibody (10498-1-AP)

    WB analysis of mouse brain using 10498-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SNCB Polyclonal antibody (10498-1-AP)

    WB analysis of human brain using 10498-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SNCB Polyclonal antibody (10498-1-AP)

    WB analysis of rat brain using 10498-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SNCB Polyclonal antibody (10498-1-AP)

    IP experiment of mouse brain using 10498-1-AP

    IP result of anti-SNCB (IP:10498-1-AP, 4ug; Detection:10498-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNCB Polyclonal antibody (10498-1-AP)

    IHC staining of human breast cancer using 10498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10498-1-AP (SNCB antibody) at dilution of 1:10 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SNCB Polyclonal antibody (10498-1-AP)

    IF Staining of HepG2 using 10498-1-AP

    Immunofluorescent analysis of HepG2 cells, using SNCB antibody 10498-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using SNCB Polyclonal antibody (10498-1-AP)

    FC experiment of HepG2 using 10498-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug SNCB antibody (10498-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10498-1-AP targets SNCB in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNCB fusion protein Ag0772
Full Name synuclein, beta
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-18 kDa
GenBank Accession NumberBC002902
Gene Symbol SNCB
Gene ID (NCBI) 6620
RRIDAB_2270787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SNCB antibody 10498-1-APDownload protocol
IHC protocol for SNCB antibody 10498-1-APDownload protocol
IF protocol for SNCB antibody 10498-1-APDownload protocol
IP protocol for SNCB antibody 10498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols