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- KD/KO Validated
SNIP1 Polyclonal antibody
SNIP1 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14950-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
14950-1-AP targets SNIP1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SNIP1 fusion protein Ag6772 |
| Full Name | Smad nuclear interacting protein 1 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46-50 kDa |
| GenBank Accession Number | BC027040 |
| Gene Symbol | SNIP1 |
| Gene ID (NCBI) | 79753 |
| RRID | AB_2286497 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SNIP1 antibody 14950-1-AP | Download protocol |
| IF protocol for SNIP1 antibody 14950-1-AP | Download protocol |
| IP protocol for SNIP1 antibody 14950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling.
| ||
PeerJ miR-29a-3p directly targets Smad nuclear interacting protein 1 and inhibits the migration and proliferation of cervical cancer HeLa cells.
| ||
Nat Commun SNIP1 and PRC2 coordinate cell fates of neural progenitors during brain development | ||
