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  • Featured Product
  • KD/KO Validated

SNRPB Polyclonal antibody

SNRPB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16807-1-AP

Synonyms

COD, Sm B/B, Sm protein B/B, SmB/B, SmB/SmB', snRNP B, SNRPB, SNRPB1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNRPB Polyclonal antibody (16807-1-AP)

    WB analysis using 16807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16807-1-AP (SNRPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SNRPB Polyclonal antibody (16807-1-AP)

    WB analysis of Jurkat using 16807-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16807-1-AP (SNRPB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SNRPB Polyclonal antibody (16807-1-AP)

    IHC staining of human cervical cancer using 16807-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SNRPB Polyclonal antibody (16807-1-AP)

    IHC staining of human cervical cancer using 16807-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SNRPB Polyclonal antibody (16807-1-AP)

    IHC staining of human liver cancer using 16807-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SNRPB Polyclonal antibody (16807-1-AP)

    IHC staining of human liver cancer using 16807-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman cervical cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16807-1-AP is SNRPB Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRPB fusion protein Ag10298
Full Name small nuclear ribonucleoprotein polypeptides B and B1
Calculated Molecular Weight234aa,25 kDa; 285aa,30 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC003530
Gene Symbol SNRPB
Gene ID (NCBI) 6628
RRIDAB_2878319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SNRPB antibody 16807-1-APDownload protocol
IHC protocol for SNRPB antibody 16807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Elife

Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation.

Authors - Maxim I Maron
View Article
humanIHC

Cell Death Dis

SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.

Authors - Nianli Liu
  • KO Validated
View Article
humanWB,IHC

Oncogene

The splicing factor SNRPB promotes ovarian cancer progression through regulating aberrant exon skipping of POLA1 and BRCA2

Authors - Yingwei Li
  • KD Validated
View Article
human,mouseWB,IHC

Aging (Albany NY)

SNRPB promotes the progression of hepatocellular carcinoma via regulating cell cycle, oxidative stress, and ferroptosis

Authors - Xiaoyan Wang
View Article