• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SNRPD2 Polyclonal antibody

SNRPD2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14789-1-AP

Synonyms

Sm D2, SMD2, snRNP core protein D2, SNRPD2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A2780 cells using SNRPD2 Polyclonal antibody (14789-1-AP)

    WB analysis of A2780 using 14789-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using SNRPD2 Polyclonal antibody (14789-1-AP)

    WB analysis of A2780 using 14789-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SNRPD2 Polyclonal antibody (14789-1-AP)

    WB analysis of A549 using 14789-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SNRPD2 Polyclonal antibody (14789-1-AP)

    WB analysis of A549 using 14789-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using SNRPD2 Polyclonal antibody (14789-1-AP)

    WB analysis of HL-60 using 14789-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using SNRPD2 Polyclonal antibody (14789-1-AP)

    WB analysis of HL-60 using 14789-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using SNRPD2 Polyclonal antibody (14789-1-AP)

    IHC staining of human skin using 14789-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SNRPD2 Polyclonal antibody (14789-1-AP)

    IHC staining of human lung cancer using 14789-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SNRPD2 Polyclonal antibody (14789-1-AP)

    IHC staining of human lung cancer using 14789-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skin tissue using SNRPD2 Polyclonal antibody (14789-1-AP)

    IHC staining of human skin using 14789-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human lung tissue using SNRPD2 Polyclonal antibody (14789-1-AP)

    IHC staining of human lung using 14789-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14789-1-AP (SNRPD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using SNRPD2 Polyclonal antibody (14789-1-AP)

    IHC staining of human lung using 14789-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14789-1-AP (SNRPD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA2780 cells, A549 cells, HL-60 cells
Positive IHC detected inhuman skin tissue, human lung cancer tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14789-1-AP targets SNRPD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRPD2 fusion protein Ag6443
Full Name small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-16 kDa
GenBank Accession NumberBC000486
Gene Symbol SNRPD2
Gene ID (NCBI) 6633
RRIDAB_10898359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD2 is one of Sm protein, and is required for pre-mRNA splicing, snRNP biogenesis.

Protocols

Product Specific Protocols
WB protocol for SNRPD2 antibody 14789-1-APDownload protocol
IHC protocol for SNRPD2 antibody 14789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

RNA Biol

Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.

Authors - Sikandar Azam
View Article