SNRPF Polyclonal antibody

SNRPF Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14977-1-AP

Synonyms

PBSCF, Sm F, Sm protein F, SMF, snRNP F, SNRPF



Tested Applications

Positive WB detected inhuman heart tissue, HeLa cells, HepG2 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14977-1-AP targets SNRPF in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRPF fusion protein Ag6889
Full Name small nuclear ribonucleoprotein polypeptide F
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC002505
Gene Symbol SNRPF
Gene ID (NCBI) 6636
RRIDAB_2302166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 4 major small nuclear ribonucleoprotein particles (snRNPs), U1, U2, U4/U6, and U5, are essential components of the eukaryotic pre-mRNA splicing machinery. These snRNPs share 8 proteins which form the snRNP structural core: B/B-prime (SNRPB), D1 (SNRPD1), D2 (SNRPD2), D3 (SNRPD3), E (SNRPE), F (SNRPF), and G (SNRPG). These proteins have a role in the biogenesis of the snRNPs.

Protocols

Product Specific Protocols
WB protocol for SNRPF antibody 14977-1-APDownload protocol
IHC protocol for SNRPF antibody 14977-1-APDownload protocol
IF protocol for SNRPF antibody 14977-1-APDownload protocol
FC protocol for SNRPF antibody 14977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols