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SNUPN Polyclonal antibody

SNUPN Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15358-1-AP

Synonyms

KPNBL, RNA U transporter 1, RNUT1, SNUPN, snurportin 1, Snurportin1, SPN1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SNUPN Polyclonal antibody (15358-1-AP)

    WB analysis of HeLa using 15358-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15358-1-AP (SNUPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SNUPN Polyclonal antibody (15358-1-AP)

    WB analysis of HeLa using 15358-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15358-1-AP (SNUPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using SNUPN Polyclonal antibody (15358-1-AP)

    IHC staining of human brain using 15358-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SNUPN Polyclonal antibody (15358-1-AP)

    IHC staining of human brain using 15358-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SNUPN Polyclonal antibody (15358-1-AP)

    IF Staining of MCF-7 using 15358-1-AP

    Immunofluorescent analysis of MCF-7 cells, using SNUPN antibody 15358-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNUPN Polyclonal antibody (15358-1-AP)

    IF Staining of HeLa using 15358-1-AP

    Immunofluorescent analysis of HeLa cells using 15358-1-AP (SNUPN antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNUPN Polyclonal antibody (15358-1-AP)

    IF Staining of HeLa cells using 15358-1-AP

    IF result of 15358-1-AP from Dr. Ralph Kehlenbach.

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15358-1-AP targets SNUPN in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNUPN fusion protein Ag7604
Full Name snurportin 1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC004203
Gene Symbol SNUPN
Gene ID (NCBI) 10073
RRIDAB_2192073
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SNUPN antibody 15358-1-APDownload protocol
IHC protocol for SNUPN antibody 15358-1-APDownload protocol
IF protocol for SNUPN antibody 15358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Neurobiol Dis

UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.

Authors - Simona Rossi
View Article