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  • Featured Product
  • KD/KO Validated

SNW1 Polyclonal antibody

SNW1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25926-1-AP

Synonyms

Bx42, NCOA 62, Nuclear protein SkiP, Prp45, PRPF45, Ski interacting protein, SKIIP, SKIP, SNW domain containing 1, SNW1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SNW1 Polyclonal antibody (25926-1-AP)

    WB analysis of HepG2 using 25926-1-AP

    WB result of SNW1 antibody (25926-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNW1 transfected HepG2 cells.

  • Western Blot (WB) analysis of MCF-7 cells using SNW1 Polyclonal antibody (25926-1-AP)

    WB analysis of MCF-7 using 25926-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25926-1-AP (SNW1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SNW1 Polyclonal antibody (25926-1-AP)

    WB analysis of HepG2 using 25926-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25926-1-AP (SNW1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SNW1 Polyclonal antibody (25926-1-AP)

    IHC staining of human prostate cancer using 25926-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SNW1 Polyclonal antibody (25926-1-AP)

    IHC staining of human prostate cancer using 25926-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SNW1 Polyclonal antibody (25926-1-AP)

    IF Staining of HepG2 using 25926-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNW1 antibody (25926-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SNW1 Polyclonal antibody (25926-1-AP)

    IF Staining of HepG2 using 25926-1-AP

    Immunofluorescent analysis of HepG2 cells using 25926-1-AP (SNW1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25926-1-AP is SNW1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNW1 fusion protein Ag23106
Full Name SNW domain containing 1
Calculated Molecular Weight 536 aa, 61 kDa
Observed Molecular Weight 62-69 kDa
GenBank Accession NumberBC108903
Gene Symbol SNW1
Gene ID (NCBI) 22938
RRIDAB_2880298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SNW1 antibody 25926-1-APDownload protocol
IHC protocol for SNW1 antibody 25926-1-APDownload protocol
IF protocol for SNW1 antibody 25926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.

Authors - Shuai Hou
View Article
humanWB

FEBS Lett

Split-BioID: a proximity biotinylation assay for dimerization-dependent protein interactions.

Authors - Sofie De Munter
View Article
humanWB

Microbes Infect

SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection.

Authors - Qiao Zhang
  • KD Validated
View Article
mouseWB

J Ovarian Res

Alteration of the N6-methyladenosine methylation landscape in a mouse model of polycystic ovary syndrome

Authors - Lingxiao Zou
View Article