• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

SORBS1 Polyclonal antibody

SORBS1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13854-1-AP

Synonyms

c Cbl associated protein, CAP, Ponsin, R85FL, SH3 domain protein 5, SH3D5, SH3P12, SORB1, SORBS1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of 3T3-L1 cells using SORBS1 Polyclonal antibody (13854-1-AP)

    WB analysis of 3T3-L1 using 13854-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SORBS1 Polyclonal antibody (13854-1-AP)

    WB analysis of HEK-293 using 13854-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SORBS1 Polyclonal antibody (13854-1-AP)

    WB analysis of mouse kidney using 13854-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SORBS1 Polyclonal antibody (13854-1-AP)

    WB analysis of mouse liver using 13854-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SORBS1 Polyclonal antibody (13854-1-AP)

    WB analysis of mouse kidney using 13854-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SORBS1 Polyclonal antibody (13854-1-AP)

    IP experiment of mouse liver using 13854-1-AP

    IP result of anti-SORBS1 (IP:13854-1-AP, 4ug; Detection:13854-1-AP 1:800) with mouse liver tissue lysate 4800ug.

Tested Applications

Positive WB detected in3T3-L1 cells, HEK-293 cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13854-1-AP targets SORBS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SORBS1 fusion protein Ag4828
Full Name sorbin and SH3 domain containing 1
Calculated Molecular Weight 1292 aa, 142 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC042612
Gene Symbol SORBS1
Gene ID (NCBI) 10580
RRIDAB_11232224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SORBS1 antibody 13854-1-APDownload protocol
IP protocol for SORBS1 antibody 13854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB，IHC

Front Cell Dev Biol

Long Non-coding RNAs LOC100126784 and POM121L9P Derived From Bone Marrow Mesenchymal Stem Cells Enhance Osteogenic Differentiation via the miR-503-5p/SORBS1 Axis.

Authors - Yiyang Xu
  • KD Validated
View Article
mouseIF

Front Cell Neurosci

Integrative Cell Type-Specific Multi-Omics Approaches Reveal Impaired Programs of Glial Cell Differentiation in Mouse Culture Models of DM1.

Authors - Anchel González-Barriga
View Article
canineIF

PLoS One

Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells.

Authors - Karin Fredriksson-Lidman
View Article
human,mouseWB

Sci Rep

STAP-2 facilitates insulin signaling through binding to CAP/c-Cbl and regulates adipocyte differentiation

Authors - Yuichi Sekine
View Article