SOX10 Polyclonal antibody
SOX10 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10422-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse small intestine tissue, SH-SY5Y cells |
| Positive IP detected in | mouse colon tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 13 publications below |
| CoIP | See 1 publications below |
Product Information
10422-1-AP targets SOX10 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SOX10 fusion protein Ag0673 |
| Full Name | SRY (sex determining region Y)-box 10 |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC002824 |
| Gene Symbol | SOX10 |
| Gene ID (NCBI) | 6663 |
| RRID | AB_2195371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX10 antibody 10422-1-AP | Download protocol |
| IHC protocol for SOX10 antibody 10422-1-AP | Download protocol |
| IP protocol for SOX10 antibody 10422-1-AP | Download protocol |
| FC protocol for SOX10 antibody 10422-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol Machine learning-based identification of SOX10 as an immune regulator of macrophage in gliomas | ||
J Cereb Blood Flow Metab The role of serum/glucocorticoid-regulated kinase 1 in brain function following cerebral ischemia | ||
Sci Rep Schwann cell-encapsulated chitosan-collagen hydrogel nerve conduit promotes peripheral nerve regeneration in rodent sciatic nerve defect models | ||
J Mol Med (Berl) MESP2 variants contribute to conotruncal heart defects by inhibiting cardiac neural crest cell proliferation. | ||
Genes Cells TAZ inhibits acinar cell differentiation but promotes immature ductal cell proliferation in adult mouse salivary glands. |
