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CoraLite®594-conjugated SOX2 Monoclonal antibody

SOX2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4B9B10

Cat no : CL594-66411

Synonyms

ANOP3, MCOPS3, SOX2, Transcription factor SOX 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated SOX2 Monoclonal antibody (CL594-66411)

    IF Staining of mouse brain using CL594-66411

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66411 (SOX2 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CoraLite®594-conjugated SOX2 Monoclonal antibody (CL594-66411)

    IF Staining of rat brain using CL594-66411

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:400.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated SOX2 Monoclonal antibody (CL594-66411)

    IF Staining of mouse brain using CL594-66411

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:200. DAPI (blue).

  • Flow cytometry (FC) experiment of U-251 cells using CoraLite®594-conjugated SOX2 Monoclonal antibody (CL594-66411)

    FC experiment of U-251 using CL594-66411

    1X10^6 U-251 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human SOX2 (CL594-66411, Clone:4B9B10) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inmouse brain tissue, rat brain tissue
Positive FC detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66411 targets SOX2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SOX2 fusion protein Ag13635
Full Name SRY (sex determining region Y)-box 2
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34-40 kDa
GenBank Accession NumberBC013923
Gene Symbol SOX2
Gene ID (NCBI) 6657
RRIDAB_2883578
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. This antibody is raised against an internal region of human SOX2. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 SOX2 antibody CL594-66411Download protocol
Standard Protocols
Click here to view our Standard Protocols