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SP110 Polyclonal antibody

SP110 Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11502-1-AP

Synonyms

IFI41, IFI75, IPR1, SP110, SP110 nuclear body protein, Speckled 110 kDa, VODI

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SP110 Polyclonal antibody (11502-1-AP)

    WB analysis of HeLa using 11502-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SP110 Polyclonal antibody (11502-1-AP)

    WB analysis using 11502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SP110 Polyclonal antibody (11502-1-AP)

    WB analysis of Jurkat using 11502-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SP110 Polyclonal antibody (11502-1-AP)

    WB analysis of K-562 using 11502-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using SP110 Polyclonal antibody (11502-1-AP)

    WB analysis of mouse small intestine using 11502-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using SP110 Polyclonal antibody (11502-1-AP)

    WB analysis of mouse spleen using 11502-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SP110 Polyclonal antibody (11502-1-AP)

    WB analysis of PC-3 using 11502-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SP110 Polyclonal antibody (11502-1-AP)

    WB analysis of human brain using 11502-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using SP110 Polyclonal antibody (11502-1-AP)

    WB analysis of human testis using 11502-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SP110 Polyclonal antibody (11502-1-AP)

    IP experiment of HeLa using 11502-1-AP

    IP result of anti-SP110 (IP:11502-1-AP, 4ug; Detection:11502-1-AP 1:800) with HeLa cells lysate 4000ug.

  • Flow cytometry (FC) experiment of Jurkat cells using SP110 Polyclonal antibody (11502-1-AP)

    FC experiment of Jurkat using 11502-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human SP110 (11502-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inA2780 cells, PC-3 cells, human brain tissue, Jurkat cells, K-562 cells, human testis tissue, HeLa cells, COLO 320 cells.
Positive IP detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11502-1-AP targets SP110 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP110 fusion protein Ag2042
Full Name SP110 nuclear body protein
Calculated Molecular Weight 689 aa, 79 kDa
Observed Molecular Weight 62-66 kDa
GenBank Accession NumberBC019059
Gene Symbol SP110
Gene ID (NCBI) 3431
RRIDAB_10863116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SP110 antibody 11502-1-APDownload protocol
IP protocol for SP110 antibody 11502-1-APDownload protocol
FC protocol for SP110 antibody 11502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,FC

J Clin Immunol

Detection of Sp110 by Flow Cytometry and Application to Screening Patients for Veno-occlusive Disease with Immunodeficiency.

Authors - Florian A Marquardsen
View Article