SPA17 Polyclonal antibody

SPA17 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13367-1-AP

Synonyms

Cancer/testis antigen 22, CT22, SP17, SP17 1, SPA17, sperm autoantigenic protein 17, Sperm protein 17, Sperm surface protein Sp17



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13367-1-AP targets SPA17 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPA17 fusion protein Ag4186
Full Name sperm autoantigenic protein 17
Calculated Molecular Weight 151 aa, 17 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC032457
Gene Symbol SPA17
Gene ID (NCBI) 53340
RRIDAB_2194631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sperm surface protein Sp17(SPA17)is a 151 amino acids protein (17kDa), which is a sperm surface peripheral membrane protein. It is a highly conserved mammalian protein expressed in developing spermatozoa and mature spermatids, and reported to play a role in the interaction of sperm with the zona pellucida. SP17 exists as a homodimer and localizes to the head and tail of spermatozoa. SP17 has been identified as a cancer/testis antigen and is expressed in ovarian cancer and multiple myeloma. This suggests that SP17 could be suitable as a target in tumor immunotherapy.

Protocols

Product Specific Protocols
WB protocol for SPA17 antibody 13367-1-APDownload protocol
IHC protocol for SPA17 antibody 13367-1-APDownload protocol
IP protocol for SPA17 antibody 13367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Br J Cancer

Tumour antigen expression in hepatocellular carcinoma in a low-endemic western area.

Authors - K Sideras
humanWB,IF

Front Immunol

Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis.

Authors - Zewei Tu
mouseWB

Sci Rep

Testis-specific peroxiredoxin 4 variant is not absolutely required for spermatogenesis and fertility in mice.

Authors - Takujiro Homma
humanIHC

BMC Cancer

Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study.

Authors - Laurie L Brunette
humanWB

Asian J Androl

Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation

Authors - Kun Zhang