SPARCL1 Polyclonal antibody

SPARCL1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13517-1-AP

Synonyms

Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1



Tested Applications

Positive WB detected inhuman heart tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13517-1-AP targets SPARCL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPARCL1 fusion protein Ag4448
Full Name SPARC-like 1 (hevin)
Calculated Molecular Weight 664 aa, 75 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC033721
Gene Symbol SPARCL1
Gene ID (NCBI) 8404
RRIDAB_10598475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.

Protocols

Product Specific Protocols
WB protocol for SPARCL1 antibody 13517-1-APDownload protocol
IHC protocol for SPARCL1 antibody 13517-1-APDownload protocol
IP protocol for SPARCL1 antibody 13517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

CNS Neurosci Ther

Endothelial genetic deletion of CD147 induces changes in the dual function of the blood-brain barrier and is implicated in Alzheimer's disease.

Authors - Hao Wang

J Neurochem

Defective Synthesis and Release of Astrocytic Thrombospondin-1 Mediates the Neuronal TDP-43 Proteinopathy Resulting in Defects in Neuronal Integrity Associated with Chronic Traumatic Encephalopathy: In Vitro Studies.

Authors - A R Jayakumar
humanIHC

Cancer Biomark

Integrated analysis reveals down-regulation of SPARCL1 is correlated with cervical cancer development and progression

Authors - Dong-Ming Wu
humanWB

Oral Dis

FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization

Authors - Xiangrui Ma
mouseIF

PLoS One

Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility.

Authors - Ling Yu
mouseWB

J Chem Neuroanat

Olfactory ensheathing cells facilitate neurite sprouting and outgrowth by secreting high levels of hevin.

Authors - Lite Ge