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  • KD/KO Validated

SPHK1 Polyclonal antibody

SPHK1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10670-1-AP

Synonyms

SK 1, sphingosine kinase 1, SPHK, SPHK1, SPHK1 phopho, SPK, SPK 1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, MCF-7 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10670-1-AP targets SPHK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPHK1 fusion protein Ag1051
Full Name sphingosine kinase 1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC008040
Gene Symbol SPHK1
Gene ID (NCBI) 8877
RRIDAB_2195809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).

Protocols

Product Specific Protocols
WB protocol for SPHK1 antibody 10670-1-APDownload protocol
IHC protocol for SPHK1 antibody 10670-1-APDownload protocol
IP protocol for SPHK1 antibody 10670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Food Funct

The preventive effect of mussel oil on gestational diabetes mellitus in pregnant mice fed by a high-fat and high-sucrose diet

Authors - Kelei Li
human,mouseWB,IP

Cells

UCHL1 Regulates Radiation Lung Injury via Sphingosine Kinase-1

Authors - Yulia Epshtein
humanWB

Pharmacol Res

Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.

Authors - Jiale Zhu
humanIHC

Cancer Cell Int

Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer

Authors - Enkui Zhang
mouseIF

Int J Mol Sci

Sphingosine Kinases at the Intersection of Pro-Inflammatory LPS and Anti-Inflammatory Endocannabinoid Signaling in BV2 Mouse Microglia Cells

Authors - Sara Standoli
humanWB

Ecotoxicol Environ Saf

Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.

Authors - Liangjing Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (03-12-2020)

Strong and specific band at correct size for western blot.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart