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SR140 Polyclonal antibody

SR140 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21399-1-AP

Synonyms

KIAA0332, SR140, U2 associated protein SR140, U2 associated SR140 protein, U2SURP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of mouse heart using 21399-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of HeLa using 21399-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of human brain using 21399-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of L02 using 21399-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SR140 Polyclonal antibody (21399-1-AP)

    IHC staining of human breast cancer using 21399-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SR140 Polyclonal antibody (21399-1-AP)

    IHC staining of human breast cancer using 21399-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HEK-293 using 21399-1-AP

    Immunofluorescent analysis of 293 cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HeLa using 21399-1-AP

    Immunofluorescent analysis of Hela cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HepG2 using 21399-1-AP

    Immunofluorescent analysis of HepG2 cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, human brain tissue, L02 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, Hela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21399-1-AP is SR140 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SR140 fusion protein Ag16093
Full Name U2-associated SR140 protein
Calculated Molecular Weight 1029 aa, 118 kDa
Observed Molecular Weight 120-140 kDa
GenBank Accession NumberBC111692
Gene Symbol SR140
Gene ID (NCBI) 23350
RRIDAB_10863661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SR140 antibody 21399-1-APDownload protocol
IHC protocol for SR140 antibody 21399-1-APDownload protocol
IF protocol for SR140 antibody 21399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols