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  • KD/KO Validated

SRX1 Polyclonal antibody

SRX1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14273-1-AP

Synonyms

C20orf139, SRX, SRX1, SRXN1, Sulfiredoxin 1, YKL086W



Tested Applications

Positive WB detected inA549 cells, mouse liver tissue, Transfected HEK-293 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14273-1-AP targets SRX1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRX1 fusion protein Ag5613
Full Name sulfiredoxin 1 homolog (S. cerevisiae)
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC047707
Gene Symbol SRXN1
Gene ID (NCBI) 140809
RRIDAB_2195030
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SRX1 antibody 14273-1-APDownload protocol
IHC protocol for SRX1 antibody 14273-1-APDownload protocol
IP protocol for SRX1 antibody 14273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Nat Commun

Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle.

Authors - John T Olthoff
humanWB,IHC

Proc Natl Acad Sci U S A

Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.

Authors - Wei Qiou Q
humanIHC

Cancer Res

Genetic polymorphisms and protein expression of NRF2 and Sulfiredoxin predict survival outcomes in breast cancer.

Authors - Hartikainen Jaana M JM
humanWB

Oncogene

PMID: 28504720

Authors - E-J Choi
human,mouseWB,IHC

Cancer Lett

Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis.

Authors - Murli Mishra
  • KD Validated
mouseWB,IHC

Carcinogenesis

Tumor Promoter-induced Sulfiredoxin Is Required for Mouse Skin Tumorigenesis.

Authors - Lisha Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (09-23-2023)

Detecting lower molecular weight molecules in gels is relatively difficult, we tried several antibodies from different companies and this worked well at a concentration of 1:500.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Breast cancer cells
SRX1 Antibody Western Blot validation (1:500 dilution) in Breast cancer cells (Cat no:14273-1-AP)
FH

Christopher (Verified Customer) (12-03-2018)

Primary neurons were lysed in RIPA and 20ug samples (containing 1x Laemmli) were run on Bis-Tris gels in 1xMOPS buffer. This antibody recognised SRXN-1 in HEK293FT cells transfected with Human SRXN1 at 17kDa. In HEK cells, non specific bands were visible at around 28 and 35kDa as well.In primary neurons, the antibody recognised a band at roughly 15kDa however it binds non sepcifically to the Pageruler Prestained protein ladder which is an issue.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Primary cortical neurons and transfected HEK293FT cells