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SRY Monoclonal antibody

SRY Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E9A4

Cat no : 67349-1-Ig

Synonyms

sex determining region Y, SRY, TDF, TDY, Testis determining factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRY Monoclonal antibody (67349-1-Ig)

    WB analysis using 67349-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SRY negative cell line HeLa cell line was served as negative control.

  • Western Blot (WB) analysis of LNCaP cells using SRY Monoclonal antibody (67349-1-Ig)

    WB analysis of LNCaP using 67349-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using SRY Monoclonal antibody (67349-1-Ig)

    WB analysis of DU 145 using 67349-1-Ig

    DU 145 cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, DU 145 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67349-1-Ig targets SRY in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SRY fusion protein Ag12610
Full Name sex determining region Y
Calculated Molecular Weight 204 aa, 24 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC074924
Gene Symbol SRY
Gene ID (NCBI) 6736
RRIDAB_2882607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.

Protocols

Product Specific Protocols
WB protocol for SRY antibody 67349-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Recruitment of DNA to tumor-derived microvesicles.

Authors - James W Clancy
View Article