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ST3GAL2 Polyclonal antibody

ST3GAL2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13620-1-AP

Synonyms

Alpha 2,3 ST 2, Gal NAc6S, Sialyltransferase 4B, SIAT4 B, SIAT4B, ST3Gal II, ST3GAL2, ST3GalA.2, ST3GALII

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using ST3GAL2 Polyclonal antibody (13620-1-AP)

    WB analysis of human colon using 13620-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ST3GAL2 Polyclonal antibody (13620-1-AP)

    WB analysis of human heart using 13620-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ST3GAL2 Polyclonal antibody (13620-1-AP)

    WB analysis of K-562 using 13620-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using ST3GAL2 Polyclonal antibody (13620-1-AP)

    WB analysis of human spleen using 13620-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ST3GAL2 Polyclonal antibody (13620-1-AP)

    WB analysis of HL-60 using 13620-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ST3GAL2 Polyclonal antibody (13620-1-AP)

    WB analysis of human liver using 13620-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat heart tissue using ST3GAL2 Polyclonal antibody (13620-1-AP)

    IHC staining of rat heart using 13620-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using ST3GAL2 Polyclonal antibody (13620-1-AP)

    IHC staining of rat heart using 13620-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using ST3GAL2 Polyclonal antibody (13620-1-AP)

    IHC staining of human skin using 13620-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using ST3GAL2 Polyclonal antibody (13620-1-AP)

    IHC staining of human skin using 13620-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inhuman colon tissue, HL-60 cells, human heart tissue, human liver tissue, human spleen tissue, K-562 cells
Positive IHC detected inrat heart tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13620-1-AP targets ST3GAL2 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST3GAL2 fusion protein Ag4528
Full Name ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Calculated Molecular Weight 350 aa, 40 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC036777
Gene Symbol ST3GAL2
Gene ID (NCBI) 6483
RRIDAB_2286561
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) is a sialyltransferase (ST) that transfers sialic acid from the activated donor CMP-sialic acid to the glycan terminus of a glycolipid or glycoprotein. ST3GAL2 contains two putative N-glycosylation sites (Asn92 and Asn211), which are mainly responsible for GD1a and GT1b ganglioside biosynthesis in the brain. ST3GAL2 belongs to the sialyltransferase family, which mediates the terminal modification of glycoproteins and glycolipids. ST3GAL2 has been found to play a role in obesity, aging, and malignant diseases (PMID: 34319453). The calculated molecular weight of ST3GAL2 is 40 kDa, but the observed is 55 kDa, which may be the glycosylation form of ST3GAL2.

Protocols

Product Specific Protocols
WB protocol for ST3GAL2 antibody 13620-1-APDownload protocol
IHC protocol for ST3GAL2 antibody 13620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols