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ST6GALNAC6 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66209-1-Ig |CloneNo.: 1A7B7

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Con: 111 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

ST6GALNAC6 Antibody WB COLO 320 cells 66209-1-Ig

COLO 320 cells were subjected to SDS PAGE followed by western blot with 66209-1-Ig( ST6GALNAC6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ST6GALNAC6 Antibody IHC mouse colon tissue 66209-1-Ig

Immunohistochemistry of paraffin-embedded mouse colon tissue slide using 66209-1-Ig( ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ST6GALNAC6 Antibody IHC mouse colon tissue 66209-1-Ig

Immunohistochemistry of paraffin-embedded mouse colon tissue slide using 66209-1-Ig( ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse
Positive WB detected in:
COLO 320 cells
Positive IHC detected in:
mouse colon tissue, human skin tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ST6GALNAC6 fusion protein Ag8358
Full name:
ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
Calculated molecular weight:
333aa,38 kDa;374aa,41 kDa
Observed molecular weight:
38-42 kDa
GenBank accession number:
BC007802
Gene ID (NCBI):
30815
Gene symbol
ST6GALNAC6
Synonyms
hST6GalNAc VI, RP11 203J24.3, Sialyltransferase 7F, SIAT7 F, SIAT7F, ST6GalNAc VI, ST6GALNAC6, ST6GALNACVI

Background

ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.


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