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ST6GALNAC6 Monoclonal antibody

ST6GALNAC6 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A7B7

Cat no : 66209-1-Ig

Synonyms

hST6GalNAc VI, RP11 203J24.3, Sialyltransferase 7F, SIAT7 F, SIAT7F, ST6GalNAc VI, ST6GALNAC6, ST6GALNACVI

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    WB analysis of COLO 320 using 66209-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IHC staining of mouse colon using 66209-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IHC staining of mouse colon using 66209-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IHC staining of human skin using 66209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IHC staining of human skin using 66209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IF Staining of human colon cancer using 66209-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ST6GALNAC6 antibody (66209-1-Ig, Clone: 1A7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inmouse colon tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66209-1-Ig targets ST6GALNAC6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ST6GALNAC6 fusion protein Ag8358
Full Name ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
Calculated Molecular Weight333aa,38 kDa; 374aa,41 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC007802
Gene Symbol ST6GALNAC6
Gene ID (NCBI) 30815
RRIDAB_2881600
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.

Protocols

Product Specific Protocols
WB protocol for ST6GALNAC6 antibody 66209-1-IgDownload protocol
IHC protocol for ST6GALNAC6 antibody 66209-1-IgDownload protocol
IF protocol for ST6GALNAC6 antibody 66209-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols