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STAT3 Polyclonal antibody

STAT3 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

ELISA

Conjugate

Unconjugated

Cat no : 10025-2-AP

Synonyms

Acute phase response factor, APRF, HIES, STAT3

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using STAT3 Polyclonal antibody (10025-2-AP)

    WB analysis of HEK-293 using 10025-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10025-2-AP (STAT3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT3 Polyclonal antibody (10025-2-AP)

    IHC staining of human lung cancer using 10025-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10025-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT3 Polyclonal antibody (10025-2-AP)

    IHC staining of human lung cancer using 10025-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10025-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10025-2-AP targets STAT3 in ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAT3 fusion protein Ag0018
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Calculated Molecular Weight 90-100 kDa
GenBank Accession NumberBC014482
Gene Symbol STAT3
Gene ID (NCBI) 6774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against human STAT3 fusion protein.