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- KD/KO Validated
STBD1 Polyclonal antibody
STBD1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11842-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, HeLa cells, A549 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 1 publications below |
| IF | See 6 publications below |
| IP | See 2 publications below |
Product Information
11842-1-AP targets STBD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STBD1 fusion protein Ag2481 |
| Full Name | starch binding domain 1 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 35-43 kDa |
| GenBank Accession Number | BC022301 |
| Gene Symbol | STBD1 |
| Gene ID (NCBI) | 8987 |
| RRID | AB_2197523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STBD1 antibody 11842-1-AP | Download protocol |
| IHC protocol for STBD1 antibody 11842-1-AP | Download protocol |
| IP protocol for STBD1 antibody 11842-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Asiatic acid alleviates ischemic myocardial injury in mice by modulating mitophagy- and glycophagy-based energy metabolism. | ||
J Cell Sci Stbd1 promotes glycogen clustering during endoplasmic reticulum stress and supports survival of mouse myoblasts
| ||
Mol Endocrinol Transgenic muscle specific Nor-1 expression regulates multiple pathways that effect adiposity, metabolism and endurance. | ||
J Cell Sci Mouse Stbd1 is N-myristoylated and affects ER-mitochondria association and mitochondrial morphology. | ||
J Biol Chem Starch binding domain-containing protein 1/genethonin 1 is a novel participant in glycogen metabolism. |
