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KD/KO Validated

STK25 Polyclonal antibody

Name: STK25 Polyclonal antibody
Brand: Proteintech
SKU: 25821-1-AP

STK25 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 25821-1-AP

Synonyms

SOK 1, SOK1, Ste20 like kinase, STK25, YSK1

Validation Data Gallery

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AllWBIP

WB analysis using 25821-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 25821-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 25821-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 25821-1-AP
IP result of anti-STK25 (IP:25821-1-AP, 4ug; Detection:25821-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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Cat No. 25821-1-AP

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Tested Applications

Positive WB detected in	Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IP	See 1 publications below

Product Information

The immunogen of 25821-1-AP is STK25 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	STK25 fusion protein Ag22964
Full Name	serine/threonine kinase 25 (STE20 homolog, yeast)
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC015793
Gene Symbol	STK25
Gene ID (NCBI)	10494
RRID	AB_2880255
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STK25 antibody 25821-1-AP	Download protocol
IP protocol for STK25 antibody 25821-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IP	Cell Mol Gastroenterol Hepatol Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease. Authors - Emmelie Cansby View Article
human,mouse	WB	Cell Mol Gastroenterol Hepatol Antagonizing STK25 Signaling Suppresses the Development of Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory, and Pro-Oncogenic Pathways. Authors - Yeshwant Kurhe KD Validated View Article
human	WB	J Exp Clin Cancer Res STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer. Authors - Fan Wu KD Validated View Article
human	WB	Mol Carcinog Downregulation of STK25 promotes autophagy via the Janus kinase 2/signal transducer and activator of transcription 3 pathway in colorectal cancer. Authors - Jiangbo Chen View Article
rat	WB	Cell Mol Neurobiol Lanthanum Chloride Induces Axon Abnormality Through LKB1-MARK2 and LKB1-STK25-GM130 Signaling Pathways. Authors - Zeli Song View Article
mouse	WB	Biochem Biophys Res Commun Loss of serine/threonine protein kinase 25 in retinal ganglion cells ameliorates high glucose-elicited damage through regulation of the AKT-GSK-3β/Nrf2 pathway. Authors - Zhuolin Zhou KD Validated View Article

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Research Area

STK25 Polyclonal antibody

STK25 Polyclonal Antibody for IP, WB, ELISA

Validation Data Gallery

WB analysis using 25821-1-AP

WB analysis of mouse brain using 25821-1-AP

WB analysis of Neuro-2a using 25821-1-AP

IP experiment of mouse brain using 25821-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease.

Antagonizing STK25 Signaling Suppresses the Development of Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory, and Pro-Oncogenic Pathways.

STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.

Downregulation of STK25 promotes autophagy via the Janus kinase 2/signal transducer and activator of transcription 3 pathway in colorectal cancer.

Lanthanum Chloride Induces Axon Abnormality Through LKB1-MARK2 and LKB1-STK25-GM130 Signaling Pathways.

Loss of serine/threonine protein kinase 25 in retinal ganglion cells ameliorates high glucose-elicited damage through regulation of the AKT-GSK-3β/Nrf2 pathway.