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STK32B Polyclonal antibody

STK32B Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15438-1-AP

Synonyms

HSA250839, serine/threonine kinase 32B, STK32, STK32B, STKG6, YANK2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using STK32B Polyclonal antibody (15438-1-AP)

    WB analysis using 15438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15438-1-AP (STK32B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using STK32B Polyclonal antibody (15438-1-AP)

    IF Staining of U2OS using 15438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using STK32B antibody (15438-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, SH-SY5Y cells
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

15438-1-AP targets STK32B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK32B fusion protein Ag4507
Full Name serine/threonine kinase 32B
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 43-48 kDa
GenBank Accession NumberBC038238
Gene Symbol STK32B
Gene ID (NCBI) 55351
RRIDAB_2196612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STK32B antibody 15438-1-APDownload protocol
IF protocol for STK32B antibody 15438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Rep

Two parabrachial Cck neurons involved in the feedback control of thirst or salt appetite

Authors - Takashi Matsuda
View Article