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Syntaxin 12 Polyclonal antibody

Name: Syntaxin 12 Polyclonal antibody
Brand: Proteintech
SKU: 14259-1-AP

Syntaxin 12 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14259-1-AP

Synonyms

STX12, STX13, STX14, syntaxin 12

Validation Data Gallery

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WB analysis using 14259-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 14259-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14259-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14259-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14259-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14259-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14259-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 14259-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14259-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 14259-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 14259-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse brain using 14259-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14259-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 14259-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14259-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 14259-1-AP
Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HepG2 using 14259-1-AP
Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HepG2 using 14259-1-AP
1X10^6 HepG2 cells were stained with 0.2ug Syntaxin 12 antibody (14259-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

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Cat No. 14259-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, human brain tissue, human heart tissue, BxPC-3 cells, mouse heart tissue, mouse brain tissue, mouse kidney tissue
Positive IHC detected in	human lung cancer tissue, human kidney tissue, mouse brain tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells
Positive FC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:20-1:200
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 1 publications below

Product Information

14259-1-AP targets Syntaxin 12 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Syntaxin 12 fusion protein Ag5532
Full Name	syntaxin 12
Calculated Molecular Weight	32 kDa
Observed Molecular Weight	33-39 kDa, 66 kDa
GenBank Accession Number	BC046999
Gene Symbol	STX12
Gene ID (NCBI)	23673
RRID	AB_2198222
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).

Protocols

Product Specific Protocols
WB protocol for Syntaxin 12 antibody 14259-1-AP	Download protocol
IHC protocol for Syntaxin 12 antibody 14259-1-AP	Download protocol
IF protocol for Syntaxin 12 antibody 14259-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Mol Cell Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation. Authors - Lu Yubing Y KD Validated View Article
human	WB	PLoS Biol Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. Authors - Yajing Lv View Article
human	WB	Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. Authors - Nazma Malik View Article
human	WB	J Biol Chem Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome Authors - Hana Nůsková View Article

Syntaxin 12 Polyclonal antibody

Syntaxin 12 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Validation Data Gallery

WB analysis using 14259-1-AP

WB analysis of BxPC-3 using 14259-1-AP

WB analysis of mouse heart using 14259-1-AP

WB analysis of mouse heart using 14259-1-AP

WB analysis of human heart using 14259-1-AP

WB analysis of human brain using 14259-1-AP

WB analysis of human heart using 14259-1-AP

IHC staining of human lung cancer using 14259-1-AP

IHC staining of human lung cancer using 14259-1-AP

IHC staining of human pancreas using 14259-1-AP

IHC staining of human pancreas using 14259-1-AP

IHC staining of mouse brain using 14259-1-AP

IHC staining of mouse brain using 14259-1-AP

IHC staining of human kidney using 14259-1-AP

IHC staining of human kidney using 14259-1-AP

IF Staining of HepG2 using 14259-1-AP

IF Staining of HepG2 using 14259-1-AP

FC experiment of HepG2 using 14259-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.

Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome