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STXBP2 Monoclonal antibody

STXBP2 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G3A12

Cat no : 66238-1-Ig

Synonyms

Hunc18b, MUNC18 2, pp10122, STXBP2, STXBP2 protein, syntaxin binding protein 2, UNC18 2, UNC18B

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using STXBP2 Monoclonal antibody (66238-1-Ig)

    WB analysis of K-562 using 66238-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66238-1-Ig (STXBP2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    WB analysis of fetal human brain using 66238-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66238-1-Ig (STXBP2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using STXBP2 Monoclonal antibody (66238-1-Ig)

    IP experiment of K-562 using 66238-1-Ig

    IP result of anti-STXBP2 (IP:66238-1-Ig, 5ug; Detection:66238-1-Ig 1:2000) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of mouse brain using 66238-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of mouse brain using 66238-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of mouse brain using 66238-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of mouse brain using 66238-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of human lung cancer using 66238-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of human lung cancer using 66238-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STXBP2 Monoclonal antibody (66238-1-Ig)

    IF Staining of HepG2 using 66238-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66238-1-Ig(STXBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, fetal human brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66238-1-Ig targets STXBP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STXBP2 fusion protein Ag7640
Full Name syntaxin binding protein 2
Calculated Molecular Weight 593 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC002869
Gene Symbol STXBP2
Gene ID (NCBI) 6813
RRIDAB_2881627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.

Protocols

Product Specific Protocols
WB protocol for STXBP2 antibody 66238-1-IgDownload protocol
IHC protocol for STXBP2 antibody 66238-1-IgDownload protocol
IF protocol for STXBP2 antibody 66238-1-IgDownload protocol
IP protocol for STXBP2 antibody 66238-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

J Cell Biol

The septin cytoskeleton regulates natural killer cell lytic granule release.

Authors - Prasad V Phatarpekar
View Article
human,mouseWB,IP

Immunity

STING activation in platelets aggravates septic thrombosis by enhancing platelet activation and granule secretion

Authors - Mina Yang
View Article