STXBP3 Polyclonal antibody

STXBP3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13764-1-AP

Synonyms

MUNC18 3, MUNC18C, Platelet Sec1 protein, Protein unc 18 homolog 3, Protein unc 18 homolog C, PSP, STXBP3, syntaxin binding protein 3, UNC 18C, Unc18 3



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

13764-1-AP targets STXBP3 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STXBP3 fusion protein Ag4747
Full Name syntaxin binding protein 3
Calculated Molecular Weight 592 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC038099
Gene Symbol STXBP3
Gene ID (NCBI) 6814
RRIDAB_2196832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STXBP3 antibody 13764-1-APDownload protocol
IHC protocol for STXBP3 antibody 13764-1-APDownload protocol
IP protocol for STXBP3 antibody 13764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Diabetes

PMID: 20460426

Authors - Pontus Boström
mouseWB

J Biol Chem

microRNA-15b contributes to depression-like behavior in mice by affecting synaptic protein levels and function in the nucleus accumbens.

Authors - Li Guo
ratWB

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan