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SULF2 Polyclonal antibody

SULF2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12260-1-AP

Synonyms

Extracellular sulfatase Sulf 2, HSULF 2, KIAA1247, SULF2, sulfatase 2



Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12260-1-AP targets SULF2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SULF2 fusion protein Ag2915
Full Name sulfatase 2
Calculated Molecular Weight 870 aa, 96 kDa
Observed Molecular Weight 50 kDa, 130-150 kDa
GenBank Accession NumberBC020962
Gene Symbol SULF2
Gene ID (NCBI) 55959
RRIDAB_11183762
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULF2, also named as KIAA1247, belongs to the sulfatase family. It exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. SULF2 can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. SULF2 has several forms: the unglycosylated 100 kDa SULF2, the glycosylated 120-150 kDa SULF2, the cleaved 72-75 kDa and 50 kDa SULF2.

Protocols

Product Specific Protocols
WB protocol for SULF2 antibody 12260-1-APDownload protocol
IHC protocol for SULF2 antibody 12260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

J Exp Med

CXCR4 regulates Plasmodium development in mouse and human hepatocytes.

Authors - Hironori Bando
human,mouseWB,IHC,IF

Cancer Lett

SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.

Authors - Ruizhe He
  • KO Validated