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SULT1A3 Polyclonal antibody

SULT1A3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19499-1-AP

Synonyms

Aryl sulfotransferase 1A3/1A4, HAST, HAST3, M PST, ST1A3/ST1A4, ST1A5, STM, Sulfotransferase 1A3/1A4, SULT1A3, SULT1A4, TL PST

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis using 19499-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of COLO 320 using 19499-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of Caco-2 using 19499-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of MCF-7 using 19499-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of HepG2 using 19499-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of mouse liver using 19499-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of HT-29 using 19499-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of HuH-7 using 19499-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    IP experiment of COLO 320 using 19499-1-AP

    IP result of anti-SULT1A3 (IP:19499-1-AP, 4ug; Detection:19499-1-AP 1:200) with COLO 320 cells lysate 2240ug.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human hepatocirrhosis using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human hepatocirrhosis using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human breast cancer using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human breast cancer using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human breast cancer using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human breast cancer using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHT-29 cells, HepG2 cells, HuH-7 cells, Caco-2 cells, MCF-7 cells, mouse liver tissue, COLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19499-1-AP targets SULT1A3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberNM_177552
Gene Symbol SULT1A3
Gene ID (NCBI) 6818
RRIDAB_10642560
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT1A3, also named as STM and SULT1A4, belongs to the sulfotransferase 1 family. SULT1A3 catalyzes the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.

Protocols

Product Specific Protocols
WB protocol for SULT1A3 antibody 19499-1-APDownload protocol
IHC protocol for SULT1A3 antibody 19499-1-APDownload protocol
IP protocol for SULT1A3 antibody 19499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Lett

Expression of sulfotransferases and sulfatases in human breast cancer: impact on resveratrol metabolism.

Authors - Miksits Michaela M
View Article
mouseWB

J Adv Res

Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling.

Authors - Danyang Tian
View Article