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- KD/KO Validated
SULT1E1 Polyclonal antibody
SULT1E1 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12522-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human lung tissue, human adrenal gland tissue, human kidney tissue, human liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 14 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
12522-1-AP targets SULT1E1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SULT1E1 fusion protein Ag3216 |
| Full Name | sulfotransferase family 1E, estrogen-preferring, member 1 |
| Calculated Molecular Weight | 294 aa, 35 kDa |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | BC027956 |
| Gene Symbol | SULT1E1 |
| Gene ID (NCBI) | 6783 |
| RRID | AB_2302772 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SULT1E1 antibody 12522-1-AP | Download protocol |
| IHC protocol for SULT1E1 antibody 12522-1-AP | Download protocol |
| IP protocol for SULT1E1 antibody 12522-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Am Soc Nephrol Inhibition of Estrogen Sulfotransferase (SULT1E1/EST) Ameliorates Ischemic Acute Kidney Injury in Mice. | ||
Biochem Pharmacol PXR phosphorylated at Ser350 transduces a glucose signal to repress the estrogen sulfotransferase gene in human liver cells and fasting signal in mouse livers. | ||
J Biol Chem Estrogen sulfotransferase is an oxidative stress responsive gene that gender-specifically affects liver ischemia/reperfusion injury.
| ||
Biochem Pharmacol Farnesoid X receptor regulates SULT1E1 expression through inhibition of PGC1α binding to HNF4α. | ||
