SUMF2 Polyclonal antibody

SUMF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11210-1-AP

Synonyms

pFGE, sulfatase modifying factor 2, SUMF2, UNQ1968/PRO4500



Tested Applications

Positive WB detected inA431 cells, human skin tissue, human spleen tissue
Positive IP detected inA431 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11210-1-AP targets SUMF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUMF2 fusion protein Ag1711
Full Name sulfatase modifying factor 2
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC015600
Gene Symbol SUMF2
Gene ID (NCBI) 25870
RRIDAB_2255511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SUMF2 antibody 11210-1-APDownload protocol
IHC protocol for SUMF2 antibody 11210-1-APDownload protocol
IF protocol for SUMF2 antibody 11210-1-APDownload protocol
IP protocol for SUMF2 antibody 11210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Int J Clin Exp Pathol

Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation.

Authors - Chuanfeng Fang