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  • KD/KO Validated

SUMO2/3 Polyclonal antibody

SUMO2/3 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11251-1-AP

Synonyms

HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, SUMO2/3, SUMO3, Ubiquitin like protein SMT3B



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, L02 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11251-1-AP targets SUMO2/3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUMO2/3 fusion protein Ag1778
Full Name SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11-20 kDa
GenBank Accession NumberBC016775
Gene Symbol SUMO2
Gene ID (NCBI) 6613
RRIDAB_2198405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Protocols

Product Specific Protocols
WB protocol for SUMO2/3 antibody 11251-1-APDownload protocol
IF protocol for SUMO2/3 antibody 11251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility

Authors - Ming Kuang
humanWB

Theranostics

Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.

Authors - Shuai Huang
humanIP

mSystems

Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.

Authors - Xiaoqing Liu
mouseWB

iScience

Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress

Authors - Amr R Ghanam
mouseIP

Int J Mol Med

SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary.

Authors - Jing Cao
mouseWB,IP

Aging (Albany NY)

SUMO1-regulated DBC1 promotes p53-dependent stress-induced apoptosis of lens epithelial cells

Authors - Yan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Verdiana (Verified Customer) (03-02-2023)

Good bands. Little aspecific I think but the gels we use have a high resolution and also those were lympoblastoid cells and we know they can me strange. Anyway, it is a good antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Lymphoblastic cell line
SUMO2/3 Antibody Western Blot validation (1:1000 dilution) in Lymphoblastic cell line (Cat no:11251-1-AP)
FH

Shubham (Verified Customer) (03-13-2019)

Good

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
SUMO2/3 Antibody Western Blot, validation ( dilution) in AC16 (Cat no:11251-1-AP)