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SUNC1 Polyclonal antibody

SUNC1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26434-1-AP

Synonyms

SUN3, SUNC1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using SUNC1 Polyclonal antibody (26434-1-AP)

    WB analysis of mouse testis using 26434-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26434-1-AP (SUNC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using SUNC1 Polyclonal antibody (26434-1-AP)

    IP experiment of mouse testis using 26434-1-AP

    IP result of anti-SUNC1 (IP:26434-1-AP, 4ug; Detection:26434-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SUNC1 Polyclonal antibody (26434-1-AP)

    IF Staining of HepG2 using 26434-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26434-1-AP (SUNC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26434-1-AP is SUNC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUNC1 fusion protein Ag23780
Full Name Sad1 and UNC84 domain containing 1
Observed Molecular Weight 37-41 kDa
GenBank Accession NumberBC026189
Gene Symbol SUNC1
Gene ID (NCBI) 256979
RRIDAB_2880513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUNC1 encodes SUN3 which is a member of SUN domain protein family, an evolutionary conserved family of inner nuclear membrane (INM) proteins that share a common C-terminal motif. SUN3 is mainly expressed in testis and is involved in sperm head formation.

Protocols

Product Specific Protocols
WB protocol for SUNC1 antibody 26434-1-APDownload protocol
IF protocol for SUNC1 antibody 26434-1-APDownload protocol
IP protocol for SUNC1 antibody 26434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,CoIP

J Biol Chem

The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis.

Authors - Qian Gao
View Article