SYCE1 Polyclonal antibody

SYCE1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11063-1-AP

Synonyms

C10orf94, Cancer/testis antigen 76, CT76, SYCE1



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11063-1-AP targets SYCE1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYCE1 fusion protein Ag1527
Full Name synaptonemal complex central element protein 1
Calculated Molecular Weight 315 aa, 36 kDa
Observed Molecular Weight 40-44 kDa
GenBank Accession NumberBC034821
Gene Symbol SYCE1
Gene ID (NCBI) 93426
RRIDAB_2197057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SYCE1 antibody 11063-1-APDownload protocol
IHC protocol for SYCE1 antibody 11063-1-APDownload protocol
IF protocol for SYCE1 antibody 11063-1-APDownload protocol
IP protocol for SYCE1 antibody 11063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility.

Authors - Laura Gómez-H
mouseIF

Am J Hum Genet

Homozygous mutations in C14orf39/SIX6OS1 cause non-obstructive azoospermia and premature ovarian insufficiency in humans.

Authors - Suixing Fan
mouseWB,IF

J Steroid Biochem Mol Biol

Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells.

Authors - Qi Wang
mouseWB,IF

Cell Rep

Male fertility in mice requires classical and nonclassical androgen signaling.

Authors - Paul S Cooke