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  • Featured Product
  • KD/KO Validated

SYNCRIP Polyclonal antibody

SYNCRIP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14024-1-AP

Synonyms

GRY RBP, hnRNP Q, HNRPQ, HNRPQ1, NS1 associated protein 1, NSAP1, pp68, RP1 3J17.2, SYNCRIP

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using SYNCRIP Polyclonal antibody (14024-1-AP)

    WB analysis of A431 using 14024-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SYNCRIP Polyclonal antibody (14024-1-AP)

    WB analysis using 14024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SYNCRIP Polyclonal antibody (14024-1-AP)

    WB analysis using 14024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SYNCRIP Polyclonal antibody (14024-1-AP)

    WB analysis of HEK-293 using 14024-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SYNCRIP Polyclonal antibody (14024-1-AP)

    WB analysis of Jurkat using 14024-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SYNCRIP Polyclonal antibody (14024-1-AP)

    WB analysis of A431 using 14024-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using SYNCRIP Polyclonal antibody (14024-1-AP)

    IP experiment of A431 using 14024-1-AP

    IP result of anti-SYNCRIP (IP:14024-1-AP, 4ug; Detection:14024-1-AP 1:500) with A431 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SYNCRIP Polyclonal antibody (14024-1-AP)

    IHC staining of human colon cancer using 14024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14024-1-AP (SYNCRIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SYNCRIP Polyclonal antibody (14024-1-AP)

    IHC staining of human colon cancer using 14024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14024-1-AP (SYNCRIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SYNCRIP Polyclonal antibody (14024-1-AP)

    IF Staining of A431 using 14024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SYNCRIP antibody (14024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, Jurkat cells, HeLa cells
Positive IP detected inA431 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14024-1-AP targets SYNCRIP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYNCRIP fusion protein Ag5167
Full Name synaptotagmin binding, cytoplasmic RNA interacting protein
Calculated Molecular Weight 623 aa, 70 kDa
Observed Molecular Weight 64-74 kDa
GenBank Accession NumberBC032643
Gene Symbol SYNCRIP
Gene ID (NCBI) 10492
RRIDAB_2200601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptotagmin-binding, cytoplasmic RNA-interacting protein(SYNCRIP), also identified as hnRNP Q, involves in mRNA processing mechanisms. It's component of the CRD-mediated complex that promotes MYC mRNA stability. Also it is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. As previously reported, the 65 and 74 kDa proteins corresponded to two isoforms of SYNCRIP produced from alternatively spliced mRNAs (PMID: 15340051).

Protocols

Product Specific Protocols
WB protocol for SYNCRIP antibody 14024-1-APDownload protocol
IHC protocol for SYNCRIP antibody 14024-1-APDownload protocol
IF protocol for SYNCRIP antibody 14024-1-APDownload protocol
IP protocol for SYNCRIP antibody 14024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nucleic Acids Res

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Authors - Evan P Booy
View Article
mouseWB

Sci Rep

Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression.

Authors - Silvia Parisi
  • KD Validated
View Article