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Synaptotagmin-11 Polyclonal antibody

Synaptotagmin-11 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12031-1-AP

Synonyms

KIAA0080, Synaptotagmin 11, synaptotagmin XI, Synaptotagmin-11, SYT11, SytXI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-11 Polyclonal antibody (12031-1-AP)

    WB analysis using 12031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Synaptotagmin-11 Polyclonal antibody (12031-1-AP)

    WB analysis of mouse skeletal muscle using 12031-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-11 Polyclonal antibody (12031-1-AP)

    IHC staining of mouse brain using 12031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-11 Polyclonal antibody (12031-1-AP)

    IHC staining of mouse brain using 12031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat brain tissue, mouse skeletal muscle tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12031-1-AP targets Synaptotagmin-11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptotagmin-11 fusion protein Ag2663
Full Name synaptotagmin XI
Calculated Molecular Weight 431 aa, 48 kDa
Observed Molecular Weight 48 kDa，96 kDa
GenBank Accession NumberBC013690
Gene Symbol SYT11
Gene ID (NCBI) 23208
RRIDAB_10638432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-11 antibody 12031-1-APDownload protocol
IHC protocol for Synaptotagmin-11 antibody 12031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols