Synaptophysin Monoclonal antibody

Synaptophysin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat, Rabbit and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4E12C4

Cat no : 67864-1-Ig

Synonyms

synaptophysin, SYP



Tested Applications

Positive WB detected inPC-12 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue, Neuro-2a cells, PC-12 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67864-1-Ig targets Synaptophysin in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Pig, Mouse, Rat, Rabbit
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11803
Full Name synaptophysin
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC064550
Gene Symbol SYP
Gene ID (NCBI) 6855
RRIDAB_2918622
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
WB protocol for Synaptophysin antibody 67864-1-IgDownload protocol
IHC protocol for Synaptophysin antibody 67864-1-IgDownload protocol
IF protocol for Synaptophysin antibody 67864-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Cell Neurosci

Expression of SH3 and Multiple Ankyrin Repeat Domains Protein 3 in Mouse Retina.

Authors - Yue Xu
ratWB

Psychiatry Res

The role of reelin in the pathological mechanism of depression from clinical to rodents

Authors - Kangyu Jin
human,mouseWB

Alzheimers Dement

Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose

Authors - Xu-Qiao Chen
ratIF

Neurochem Int

Photobiomodulation treatment inhibits neurotoxic astrocytic polarization and protects neurons in in vitro and in vivo stroke models

Authors - Yu Feng
mouseWB

J Agric Food Chem

Protective Effect of 20(S)-Protopanaxadiol on D-Gal-Induced Cognitively Impaired Mice Based on Its Target Protein Brain-type Creatine Kinase

Authors - Hantao Zhang
ratIF

BMC Complement Med Ther

A whole transcriptome profiling analysis for antidepressant mechanism of Xiaoyaosan mediated synapse loss via BDNF/trkB/PI3K signal axis in CUMS rats

Authors - Pan Meng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

B (Verified Customer) (01-05-2022)

Good performance in WB with a strong and clear band at 35 Kd around.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mouse brain lysate
Synaptophysin Antibody Western Blot validation (1:5000 dilution) in mouse brain lysate (Cat no:67864-1-Ig)