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TAF7 Polyclonal antibody

TAF7 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13506-1-AP

Synonyms

TAF(II)55, TAF2F, TAF7, TAFII 55, TAFII55

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAF7 Polyclonal antibody (13506-1-AP)

    WB analysis using 13506-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13506-1-AP (TAF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TAF7 Polyclonal antibody (13506-1-AP)

    WB analysis using 13506-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13506-1-AP (TAF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using TAF7 Polyclonal antibody (13506-1-AP)

    IP experiment of Jurkat using 13506-1-AP

    IP result of anti-TAF7 (IP:13506-1-AP, 4ug; Detection:13506-1-AP 1:500) with Jurkat cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TAF7 Polyclonal antibody (13506-1-AP)

    IHC staining of human cervical cancer using 13506-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13506-1-AP (TAF7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TAF7 Polyclonal antibody (13506-1-AP)

    IHC staining of human cervical cancer using 13506-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13506-1-AP (TAF7 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13506-1-AP targets TAF7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAF7 fusion protein Ag4406
Full Name TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
Calculated Molecular Weight 349 aa, 40 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC032737
Gene Symbol TAF7
Gene ID (NCBI) 6879
RRIDAB_2271400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF7, also named as Transcription initiation factor TFIID 55 kDa subunit, is a 349 amino acid protein, which belongs to the TAF7 family. TAF7 functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF7 is phosphorylated by CIITA. The calcualted molecular weight of TAF7 is 40 kDa, but phosphorylated TAF7 is about 55kDa. (PMID: 22711989)

Protocols

Product Specific Protocols
WB protocol for TAF7 antibody 13506-1-APDownload protocol
IHC protocol for TAF7 antibody 13506-1-APDownload protocol
IP protocol for TAF7 antibody 13506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols