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  • Featured Product
  • KD/KO Validated

TALDO1 Polyclonal antibody

TALDO1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12376-1-AP

Synonyms

TAL, TAL H, TALDO, TALDO1, TALDOR, TALH, Transaldolase, transaldolase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TALDO1 Polyclonal antibody (12376-1-AP)

    WB analysis using 12376-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12376-1-AP (TALDO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TALDO1 Polyclonal antibody (12376-1-AP)

    WB analysis of HEK-293 using 12376-1-AP

    WB result of TALDO1 antibody (12376-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TALDO1 transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using TALDO1 Polyclonal antibody (12376-1-AP)

    IP experiment of COLO 320 using 12376-1-AP

    IP result of anti-TALDO1 (IP:12376-1-AP, 4ug; Detection:12376-1-AP 1:700) with COLO 320 cells lysate 3200 ug.

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, COLO 320 cells, HepG2 cells
Positive IP detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12376-1-AP targets TALDO1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TALDO1 fusion protein Ag3031
Full Name transaldolase 1
Calculated Molecular Weight 337 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC009680
Gene Symbol TALDO1
Gene ID (NCBI) 6888
RRIDAB_2199712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TALDO1 belongs to the transaldolase family which contributes to the generation of NADPH in the nonoxidative phase of the pentose phosphate pathway (PPP). Defects in TALDO1 are the cause of transaldolase 1 deficiency which results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris.

Protocols

Product Specific Protocols
WB protocol for TALDO1 antibody 12376-1-APDownload protocol
IP protocol for TALDO1 antibody 12376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Science

mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.

Authors - Issam Ben-Sahra
View Article
humanWB

Cancer Cell

Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.

Authors - Yoichiro Mitsuishi
View Article
humanWB

Nat Commun

Synthetic lethality between HER2 and transaldolase in intrinsically resistant HER2-positive breast cancers.

Authors - Yi Ding
  • KD Validated
View Article
humanWB

Nat Metab

Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.

Authors - Diem H Tran
View Article

J Biol Chem

Metabolic reprogramming driven by EZH2 inhibition depends on cell-matrix interactions

Authors - Teresa W-M Fan
View Article