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  • Featured Product
  • KD/KO Validated

TALDO1 Monoclonal antibody

TALDO1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F5G3

Cat no : 67816-1-Ig

Synonyms

TAL, TAL H, TALDO, TALDO1, TALDOR, TALH, Transaldolase, transaldolase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TALDO1 Monoclonal antibody (67816-1-Ig)

    WB analysis using 67816-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67816-1-Ig (TALDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using TALDO1 Monoclonal antibody (67816-1-Ig)

    WB analysis of HEK-293 using 67816-1-Ig

    WB result of TALDO1 antibody (67816-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TALDO1 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using TALDO1 Monoclonal antibody (67816-1-Ig)

    IHC staining of human oesophagus cancer using 67816-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67816-1-Ig (TALDO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TALDO1 Monoclonal antibody (67816-1-Ig)

    IHC staining of human prostate cancer using 67816-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67816-1-Ig (TALDO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using TALDO1 Monoclonal antibody (67816-1-Ig)

    IHC staining of human oesophagus cancer using 67816-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67816-1-Ig (TALDO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman oesophagus cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67816-1-Ig targets TALDO1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TALDO1 fusion protein Ag3031
Full Name transaldolase 1
Calculated Molecular Weight 337 aa, 38 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC009680
Gene Symbol TALDO1
Gene ID (NCBI) 6888
RRIDAB_2918579
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TALDO1 belongs to the transaldolase family which contributes to the generation of NADPH in the nonoxidative phase of the pentose phosphate pathway (PPP). Defects in TALDO1 are the cause of transaldolase 1 deficiency which results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris.

Protocols

Product Specific Protocols
WB protocol for TALDO1 antibody 67816-1-IgDownload protocol
IHC protocol for TALDO1 antibody 67816-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Nutr

Erythritol synthesis is elevated in response to oxidative stress and regulated by the non-oxidative pentose phosphate pathway in A549 cells

Authors - Semira R. Ortiz
  • KD Validated
View Article