• Featured Product
  • KD/KO Validated

TARS2 Polyclonal antibody

TARS2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15067-1-AP

Synonyms

TARS2, TARSL1, Threonine tRNA ligase, ThrRS



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse heart tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15067-1-AP targets TARS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TARS2 fusion protein Ag7074
Full Name threonyl-tRNA synthetase 2, mitochondrial (putative)
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC000541
Gene Symbol TARS2
Gene ID (NCBI) 80222
RRIDAB_2200668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TARS2 antibody 15067-1-APDownload protocol
IHC protocol for TARS2 antibody 15067-1-APDownload protocol
IF protocol for TARS2 antibody 15067-1-APDownload protocol
IP protocol for TARS2 antibody 15067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF, WB, IP

Mol Cell

Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.

Authors - Sung-Hoon Kim
  • KD Validated
humanWB

Bioengineered

Knockdown of mitochondrial threonyl-tRNA synthetase 2 inhibits lung adenocarcinoma cell proliferation and induces apoptosis.

Authors - Hui Tian
  • KD Validated
mouseWB

Redox Biol

Decreased expression of mitochondrial aminoacyl-tRNA synthetases causes downregulation of OXPHOS subunits in type 2 diabetic muscle

Authors - Iliana López-Soldado
humanIHC

Int J Biol Markers

Potential prognostic biomarkers of hepatocellular carcinoma based on 4D label-free quantitative proteomics analysis pilot investigation

Authors - Lida Suo