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TBC1D19 Polyclonal antibody

TBC1D19 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21085-1-AP

Synonyms

TBC1 domain family member 19, TBC1 domain family, member 19, TBC1D19

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using TBC1D19 Polyclonal antibody (21085-1-AP)

    WB analysis of mouse brain using 21085-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21085-1-AP (TBC1D19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using TBC1D19 Polyclonal antibody (21085-1-AP)

    WB analysis of rat brain using 21085-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 21085-1-AP (TBC1D19 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TBC1D19 Polyclonal antibody (21085-1-AP)

    WB analysis of mouse brain using 21085-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21085-1-AP (TBC1D19 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TBC1D19 Polyclonal antibody (21085-1-AP)

    IHC staining of human pancreas cancer using 21085-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21085-1-AP (TBC1D19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TBC1D19 Polyclonal antibody (21085-1-AP)

    IHC staining of human pancreas cancer using 21085-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21085-1-AP (TBC1D19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TBC1D19 Polyclonal antibody (21085-1-AP)

    IF Staining of HepG2 using 21085-1-AP

    Immunofluorescent analysis of HepG2 cells using 21085-1-AP (TBC1D19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21085-1-AP is TBC1D19 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D19 fusion protein Ag15323
Full Name TBC1 domain family, member 19
Calculated Molecular Weight 526 aa, 60 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC031642
Gene Symbol TBC1D19
Gene ID (NCBI) 55296
RRIDAB_2878809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of TBC1D19 remains largely unknown. TBC1D19 may act as a GTPase-activating protein for Rab family protein(s).

Protocols

Product Specific Protocols
WB protocol for TBC1D19 antibody 21085-1-APDownload protocol
IHC protocol for TBC1D19 antibody 21085-1-APDownload protocol
IF protocol for TBC1D19 antibody 21085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols