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TBC1D25 Monoclonal antibody

TBC1D25 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C7H3

Cat no : 67574-1-Ig

Synonyms

MG81, OATL1, TBC1 domain family member 25, TBC1 domain family, member 25, TBC1D25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D25 Monoclonal antibody (67574-1-Ig)

    WB analysis using 67574-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67574-1-Ig (TBC1D25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using TBC1D25 Monoclonal antibody (67574-1-Ig)

    WB analysis using 67574-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67574-1-Ig (TBC1D25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TBC1D25 Monoclonal antibody (67574-1-Ig)

    IF Staining of HEK-293 using 67574-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TBC1D25 antibody (67574-1-Ig, Clone: 2C7H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, Neuro-2a cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67574-1-Ig targets TBC1D25 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TBC1D25 fusion protein Ag29470
Full Name TBC1 domain family, member 25
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC101817
Gene Symbol TBC1D25
Gene ID (NCBI) 4943
RRIDAB_2882787
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TBC1D25 antibody 67574-1-IgDownload protocol
IF protocol for TBC1D25 antibody 67574-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC,WB,IF

In Vitro Cell Dev Biol Anim

TBC1 domain family member 25 protects against myocardial apoptosis and the proinflammatory response triggered by ischemia-reperfusion injury through suppression of the TAK1-JNK/p38 MAPK signaling cascade

Authors - Ziwen Liu
View Article