TBCD Polyclonal antibody

Name: TBCD Polyclonal antibody
Brand: Proteintech
SKU: 14867-1-AP

TBCD Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14867-1-AP

Synonyms

Beta tubulin cofactor D, KIAA0988, SSD 1, SSD1, TBCD, tfcD, tubulin folding cofactor D, Tubulin specific chaperone D

Validation Data Gallery

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AllWBIP

WB analysis using 14867-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14867-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14867-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse brain using 14867-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14867-1-AP
IP result of anti-TBCD (IP:14867-1-AP, 4ug; Detection:14867-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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Cat No. 14867-1-AP

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Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, MCF-7 cells
Positive IP detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 2 publications below

Product Information

14867-1-AP targets TBCD in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TBCD fusion protein Ag6310
Full Name	tubulin folding cofactor D
Calculated Molecular Weight	133 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	BC003094
Gene Symbol	TBCD
Gene ID (NCBI)	6904
RRID	AB_2199548
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TBCD antibody 14867-1-AP	Download protocol
IP protocol for TBCD antibody 14867-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	ACS Chem Neurosci Proteomic Study on the Mechanism of Arsenic Neurotoxicity in the Rat Cerebral Cortex and the Protective Mechanism of Dictyophora Polysaccharides against Arsenic Neurotoxicity Authors - Xinglai Zhang View Article
human	IF	Genes Chromosomes Cancer MYCN overexpression is associated with unbalanced copy number gain, altered nuclear location, and overexpression of chromosome arm 17q genes in neuroblastoma tumors and cell lines. Authors - Alexandra Kuzyk View Article
mouse	IF	Histochem Cell Biol Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice. Authors - Lukas Juergens View Article
human	WB	Front Genet Target-Sequencing of Female Infertility Pathogenic Gene Panel and a Novel TUBB8 Loss-of-Function Mutation. Authors - Hongxia Yuan View Article

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TBCD Polyclonal antibody

TBCD Polyclonal Antibody for IP, WB, ELISA

Validation Data Gallery

WB analysis using 14867-1-AP

WB analysis of mouse brain using 14867-1-AP

WB analysis of MCF-7 using 14867-1-AP

WB analysis of mouse brain using 14867-1-AP

IP experiment of mouse brain using 14867-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Proteomic Study on the Mechanism of Arsenic Neurotoxicity in the Rat Cerebral Cortex and the Protective Mechanism of Dictyophora Polysaccharides against Arsenic Neurotoxicity

MYCN overexpression is associated with unbalanced copy number gain, altered nuclear location, and overexpression of chromosome arm 17q genes in neuroblastoma tumors and cell lines.

Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.

Target-Sequencing of Female Infertility Pathogenic Gene Panel and a Novel TUBB8 Loss-of-Function Mutation.