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KD/KO Validated

TBK1 Polyclonal antibody

Name: TBK1 Polyclonal antibody
Brand: Proteintech
SKU: 28397-1-AP

TBK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (6)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 28397-1-AP

Synonyms

FLJ11330, NAK, NF kappa B activating kinase, T2K, TANK binding kinase 1, TBK1

Validation Data Gallery

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AllWBIPIHC

WB analysis using 28397-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28397-1-AP (TBK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 28397-1-AP
WB result of TBK1 antibody (28397-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and TBK1 knockout HeLa cells.
WB analysis using 28397-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28397-1-AP (TBK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of U2OS using 28397-1-AP
U2OS lysates prepared and IP of TBK1 performed using 1.0 μg of 28397-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IHC staining of human liver cancer using 28397-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 28397-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 28397-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 28397-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 28397-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 28397-1-AP

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Tested Applications

Positive WB detected in	HT-1080 cells, HCT 116 cells, HeLa cells, HepG2 cells, U2OS cells
Positive IHC detected in	human stomach cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 34 publications below
IHC	See 1 publications below

Product Information

28397-1-AP targets TBK1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Cited Reactivity	human, mouse, rat, chicken, monkey, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TBK1 fusion protein Ag28894
Full Name	TANK-binding kinase 1
Observed Molecular Weight	84 kDa
GenBank Accession Number	BC034950
Gene Symbol	TBK1
Gene ID (NCBI)	29110
RRID	AB_2881132
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.

Protocols

Product Specific Protocols
WB protocol for TBK1 antibody 28397-1-AP	Download protocol
IHC protocol for TBK1 antibody 28397-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Sci Adv Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation Authors - Wei Xiang View Article
human,mouse	WB	Mol Ther STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease Authors - Li Gao View Article
human	WB	Protein Cell Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response. Authors - Mi Li View Article
mouse	WB	J Neuroinflammation Stimulator of IFN genes mediates neuroinflammatory injury by suppressing AMPK signal in experimental subarachnoid hemorrhage. Authors - Yucong Peng View Article
human	WB	J Virol African Swine Fever Virus H240R Protein Inhibits the Production of Type I Interferon through Disrupting the Oligomerization of STING Authors - Guangqiang Ye View Article
human	WB,IHC	Front Immunol TANK-Binding Kinase 1 (TBK1) Serves as a Potential Target for Hepatocellular Carcinoma by Enhancing Tumor Immune Infiltration. Authors - Yuchuan Jiang View Article

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TBK1 Polyclonal antibody

TBK1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 28397-1-AP

WB analysis of HeLa using 28397-1-AP

WB analysis using 28397-1-AP

IP experiment of U2OS using 28397-1-AP

IHC staining of human liver cancer using 28397-1-AP

IHC staining of human stomach cancer using 28397-1-AP

IHC staining of human liver cancer using 28397-1-AP

IHC staining of human liver cancer using 28397-1-AP

IHC staining of human stomach cancer using 28397-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation

STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease

Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response.

Stimulator of IFN genes mediates neuroinflammatory injury by suppressing AMPK signal in experimental subarachnoid hemorrhage.

African Swine Fever Virus H240R Protein Inhibits the Production of Type I Interferon through Disrupting the Oligomerization of STING

TANK-Binding Kinase 1 (TBK1) Serves as a Potential Target for Hepatocellular Carcinoma by Enhancing Tumor Immune Infiltration.