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TBXAS1 Polyclonal antibody

TBXAS1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11321-1-AP

Synonyms

CYP5, CYP5A1, Cytochrome P450 5A1, GHOSAL, TBXAS1, THAS, Thromboxane A synthase, TS, TXA synthase, TXAS, TXS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A549 cells using TBXAS1 Polyclonal antibody (11321-1-AP)

    WB analysis of A549 using 11321-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11321-1-AP (TBXAS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using TBXAS1 Polyclonal antibody (11321-1-AP)

    WB analysis of human colon using 11321-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 11321-1-AP (TBXAS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TBXAS1 Polyclonal antibody (11321-1-AP)

    IHC staining of human lung cancer using 11321-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11321-1-AP (TBXAS1 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inA549 cells, human colon tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11321-1-AP targets TBXAS1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBXAS1 fusion protein Ag1807
Full Name thromboxane A synthase 1 (platelet)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC014117
Gene Symbol TBXAS1
Gene ID (NCBI) 6916
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in humen uterus (PMID:1386258).

Publications

SpeciesApplicationTitle
humanWB,IHC

Hypertension

Reduced methylation of the thromboxane synthase gene is correlated with its increased vascular expression in preeclampsia.

Authors - Mousa Ahmad A AA
View Article
humanIF

Respir Res

Clonally expanded human airway smooth muscle cells exhibit morphological and functional heterogeneity.

Authors - Shailendra R Singh
View Article
humanIHC,IF

Am J Physiol Lung Cell Mol Physiol

Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways?

Authors - Singh Shailendra R SR
View Article