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  • Featured Product
  • KD/KO Validated

TCEAL7 Polyclonal antibody

TCEAL7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11218-1-AP

Synonyms

MPMGp800C04260Q003, TCEA like protein 7, TCEAL7

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using TCEAL7 Polyclonal antibody (11218-1-AP)

    WB analysis of human liver using 11218-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11218-1-AP (TCEAL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TCEAL7 Polyclonal antibody (11218-1-AP)

    WB analysis of A549 using 11218-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11218-1-AP (TCEAL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TCEAL7 Polyclonal antibody (11218-1-AP)

    IHC staining of human ovary tumor using 11218-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11218-1-AP (TCEAL7 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TCEAL7 Polyclonal antibody (11218-1-AP)

    IHC staining of human gliomas using 11218-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11218-1-AP (TCEAL7 antibody) at dilution of 1:50 (under 25x lens).

Tested Applications

Positive WB detected inhuman liver tissue, A549 cells
Positive IHC detected inhuman ovary tumor tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11218-1-AP targets TCEAL7 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEAL7 fusion protein Ag1715
Full Name transcription elongation factor A (SII)-like 7
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC016786
Gene Symbol TCEAL7
Gene ID (NCBI) 56849
RRIDAB_2201131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCEAL7 is a transcription regulatory factor that has a role in the negative regulation of NF-kappa-B signaling at the basal level by regulating transcriptional activity of NF-kappa-B on its target gene promoters. It can associate with cyclin D1 promoter containing Myc E-box sequence and transcriptionally represses cyclin D1 expression. In addition, TCEAL7 regulates telomerase reverse transcriptase expression and telomerase activity in both ALT (alternative lengthening of telomeres)and telomerase-positive cell lines

Protocols

Product Specific Protocols
WB protocol for TCEAL7 antibody 11218-1-APDownload protocol
IHC protocol for TCEAL7 antibody 11218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell Int

Transcription elongation factor A-like 7, regulated by miR-758-3p inhibits the progression of melanoma through decreasing the expression levels of c-Myc and AKT1.

Authors - Xilin Liu
  • KD Validated
View Article
humanWB,IHC

PLoS One

Decreased expression of transcription elongation factor A-like 7 is associated with gastric adenocarcinoma prognosis.

Authors - Huang Chun-yu CY
View Article