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  • KD/KO Validated

TCIRG1 Polyclonal antibody

TCIRG1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12649-1-AP

Synonyms

a3, Atp6i, ATP6N1C, ATP6V0A3, OC 116 kDa, OC 116kDa, OC116, OPTB1, Stv1, T cell immune regulator 1, TCIRG1, TIRC7, V ATPase 116 kDa isoform a3, Vph1



Tested Applications

Positive WB detected inHL-60 cells, U-937 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12649-1-AP targets TCIRG1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCIRG1 fusion protein Ag3378
Full Name T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
Calculated Molecular Weight 830 aa, 93 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC018133
Gene Symbol TCIRG1
Gene ID (NCBI) 10312
RRIDAB_10644329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TCIRG1 antibody 12649-1-APDownload protocol
IF protocol for TCIRG1 antibody 12649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncotarget

PMID: 28978047

Authors - Enrica Balza
mouseIF

Cancer Med

Therapeutic efficacy of proton transport inhibitors alone or in combination with cisplatin in triple negative and hormone sensitive breast cancer models.

Authors - Enrica Balza
humanWB

Oncol Rep

TIRC7 inhibits Th1 cells by upregulating the expression of CTLA‑4 and STAT3 in mice with acute graft‑versus‑host disease.

Authors - Feng Zhu
  • KD Validated
pigWB

Front Microbiol

Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells.

Authors - Jun Hu
humanIHC,WB

Cancers (Basel)

Integrative Analysis Identifies TCIRG1 as a Potential Prognostic and Immunotherapy-Relevant Biomarker Associated with Malignant Cell Migration in Clear Cell Renal Cell Carcinoma

Authors - Chao Xu
  • KD Validated
humanWB

Cancer Cell Int

Glycolysis-related biomarker TCIRG1 participates in regulation of renal cell carcinoma progression and tumor immune microenvironment by affecting aerobic glycolysis and AKT/mTOR signaling pathway

Authors - Sichen Di