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TFAP2B Polyclonal antibody

TFAP2B Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13183-1-AP

Synonyms

AP 2B, AP2 B, AP2 beta, TFAP2B, Transcription factor AP 2 beta

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TFAP2B Polyclonal antibody (13183-1-AP)

    WB analysis of MCF-7 using 13183-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13183-1-AP (TFAP2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TFAP2B Polyclonal antibody (13183-1-AP)

    WB analysis of MCF-7 using 13183-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13183-1-AP (TFAP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using TFAP2B Polyclonal antibody (13183-1-AP)

    IP experiment of MCF-7 using 13183-1-AP

    IP result of anti-TFAP2B (IP:13183-1-AP, 4ug; Detection:13183-1-AP 1:500) with MCF-7 cells lysate 800ug.

Tested Applications

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13183-1-AP targets TFAP2B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFAP2B fusion protein Ag3853
Full Name transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
Calculated Molecular Weight 449 aa, 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC037225
Gene Symbol TFAP2B
Gene ID (NCBI) 7021
RRIDAB_2286992
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TFAP2B antibody 13183-1-APDownload protocol
IP protocol for TFAP2B antibody 13183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

miR-142-5p promotes renal cell tumorigenesis by targeting TFAP2B.

Authors - Maoshu Zhu
View Article