• Featured Product
  • KD/KO Validated

TGM2 Polyclonal antibody

TGM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15100-1-AP

Synonyms

G ALPHA h, GNAH, TG(C), TG2, TGase 2, TGase C, TGase H, TGC, TGM2, Tissue transglutaminase, Transglutaminase 2, Transglutaminase C, Transglutaminase H



Tested Applications

Positive WB detected inC2C12 cells, HUVEC cells, HepG2 cells, BxPC-3 cells, HeLa cells, A549 cells, K-562 cells, SGC-7901 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15100-1-AP targets TGM2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGM2 fusion protein Ag7439
Full Name transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 77-80 kDa
GenBank Accession NumberBC003551
Gene Symbol TGM2
Gene ID (NCBI) 7052
RRIDAB_2202885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TGM2 antibody 15100-1-APDownload protocol
IHC protocol for TGM2 antibody 15100-1-APDownload protocol
IP protocol for TGM2 antibody 15100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nature

Autoimmune amelogenesis imperfecta in patients with APS-1 and coeliac disease

Authors - Yael Gruper
humanWB,IF

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
humanWB,IF

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
humanWB

Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Authors - Chunqing Wang
humanWB,IHC

Cell Oncol (Dordr)

Transglutaminases are oncogenic biomarkers in human cancers and therapeutic targeting of TGM2 blocks chemoresistance and macrophage infiltration in pancreatic cancer

Authors - Shan Zhang
  • KD Validated
humanWB

Int J Mol Sci

TGFβ-1 Induced Cross-Linking of the Extracellular Matrix of Primary Human Dermal Fibroblasts.

Authors - Mariya E Semkova

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jon (Verified Customer) (02-15-2022)

DILuted as suggested in %5 milk TBST blocking buffer. We have had problems with other TGM2 antibodies from other companies, but this one seemed no work better and yield a better signal. However, the apparent molecular weight of the TGM2 band appeared to be at a much lower MW than it is supposed to.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: EndoCßH1 human pancreatic beta cell line
TGM2 Antibody Western Blot validation (1:1000 dilution) in EndoCßH1 human pancreatic beta cell line (Cat no:15100-1-AP)