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TGM4 Polyclonal antibody

TGM4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15847-1-AP

Synonyms

Fibrinoligase, hTGP, Prostate transglutaminase, TG(P), TGase 4, TGase P, TGM4, TGP, Transglutaminase 4, transglutaminase 4 (prostate), Transglutaminase P

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TGM4 Polyclonal antibody (15847-1-AP)

    WB analysis of mouse testis using 15847-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TGM4 Polyclonal antibody (15847-1-AP)

    WB analysis using 15847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TGM4 Polyclonal antibody (15847-1-AP)

    WB analysis of human testis using 15847-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human testis using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human testis using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human brain using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human brain using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human kidney using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human kidney using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human placenta using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human placenta using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human ovary using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using TGM4 Polyclonal antibody (15847-1-AP)

    IHC staining of human ovary using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, HeLa cells, PC-3 cells, rat testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15847-1-AP targets TGM4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGM4 fusion protein Ag8608
Full Name transglutaminase 4 (prostate)
Calculated Molecular Weight 684 aa, 77 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC007003
Gene Symbol TGM4
Gene ID (NCBI) 7047
RRIDAB_2287328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TGM4 antibody 15847-1-APDownload protocol
IHC protocol for TGM4 antibody 15847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols